• Human ATG5 ELISA Kit

Human ATG5 ELISA Kit

Date : April 6, 2022
Products & Services : Lifes cience research test Kits: Antibodies - ELISA - Protein
Supply Chain : Manufacturer, Producer

Description

:   ATG5 ELISA Kit, Autophagy protein 5 ELISA Kit, APG5-like ELISA Kit, Apoptosis-specific protein ELISA Kit, APG5L ELISA Kit, ASP ELISA Kit, hAPG5 ELISA Kit, ATG5 autophagy related 5 homolog ELISA Kit

Catalog NO.

FY-EH3593

Reactivity

Human

Assay range

0.313-20ng/ml

Sensitivity

0.188ng/ml

Product Name

Human ATG5(Autophagy protein 5) ELISA Kit

Catalog NO.

FY-EH3593

Alias

ATG5 ELISA Kit, Autophagy protein 5 ELISA Kit, APG5-like ELISA Kit, Apoptosis-specific protein ELISA Kit, APG5L ELISA Kit, ASP ELISA Kit, hAPG5 ELISA Kit, ATG5 autophagy related 5 homolog ELISA Kit

Sandwich ELISA, Double Antibody

Application

ATG5 ELISA Kit allows for the in vitro quantitative determination of ATG5 concentrations in serum, plasma, tissue homogenates and other biological fluids.

48T, 96T

Storage

2-8 ℃ for 6 months

Sensitivity

0.188ng/ml

Species

Human

UniProt ID

Q9H1Y0

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

Feiyue’s Human ATG5(Autophagy protein 5) ELISA Kit  is an ELISA reagent for detection of ATG5(Autophagy protein 5), plasma or cell with sensitivity, specificity and consistency.

AST(Aspartate Aminotransferase) Introduction

Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT). AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.

This kit uses sandwich ELISA to detect the concentration of AST(Aspartate Aminotransferase).AST(Aspartate Aminotransferase)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional AST(Aspartate Aminotransferase) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of AST(Aspartate Aminotransferase) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

 

Reference

1.Karmen A, Wroblewski F, Ladue JS (January 1955). “Transaminase activity in human blood”. The Journal of Clinical Investigation. 34 (1): 126–31. doi:10.1172/JCI103055. PMC 438594. PMID 13221663.

 

2. Giannini EG, Testa R, Savarino V (February 2005). “Liver enzyme alteration: a guide for clinicians”. Canadian Medical Association Journal. 172 (3): 367–79. doi:10.1503/cmaj.1040752. PMC 545762. PMID 15684121. Aminotransferase clearance is carried out within the liver by sinusoidal cells. The half-life in the circulation is about 47 hours for ALT, about 17 hours for total AST and, on average, 87 hours for mitochondrial AST.

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