LIF. Recombinant LIF has been produced in plants by InVitria.
2. What is the structure of LIF?
As shown in Figure 1, the human and mouse LIF genes are located on chromosomes 22q14 and 11A1-A2, respectively, with lengths of 6.0 and 6.3 kb, containing three exons of 61/3, 592/3, and 136 amino acids, the coding region sequence is highly conserved, and the homology is 78-94%. Intramolecular disulfide bonds play an important role in maintaining the structure and biological activity of LIF molecules. The molecular weight and charge of LIF vary according to the degree of glycosylation, with a molecular weight of 38-64 kDa and an IP of 8.6-9.2. The biological function of LIF in vitro appears to be independent of the degree of glycosylation, but whether glycosylation affects LIF stability and function in vivo remains to be determined. Human and mouse LIF have 78% homology at the amino acid level, and human LIF has similar activity on mouse-derived cells, while mouse LIF has a weaker effect on human cells.
In conclude, LIF is a widely expressed cytokine with distinct glycosylation patterns. Moreover, LIF receptors are present in many different tissues, including liver, central nervous system, macrophages, bone, kidney, placenta, the ICM of embryos, and the uterine epithelium. LIF can phosphorylate STAT3 through several reactions. Then, phosphorylated STAT3 dimers and translocates into the nucleus and activates target gene tranion. LIF-JAK/STAT3 is a well-established signaling pathway in pancreatic ductal adenocarcinoma (PDAC), CRC, ovarian cancer, and non-small lung cell carcinoma (NSCLC). In addition, LIF has also been shown to activate other signaling pathways, such as YAP/TEAD and AKT/mTOR.
FY-EP4040 Porcine LIF(Leukemia Inhibitory Factor) ELISA Kit
FY-EH4766 Human LIF(Leukemia Inhibitory Factor) ELISA Kit
1, GRCh38: Ensembl release 89: ENSG00000128342 – Ensembl, May 2017
2, GRCm38: Ensembl release 89: ENSMUSG00000034394 – Ensembl, May 2017
3, “Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
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